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Published by Birkhäuser, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: WeSavings LLC, MONTGOMERY, TX, U.S.A.
Book
Paperback. Condition: Good. No Jacket. X-Library book. Typical library markings. Ships promptly from Texas. 100% Money-Back Guarantee!!!.
Published by Birkhäuser Boston, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: Better World Books, Mishawaka, IN, U.S.A.
Book First Edition
Condition: Good. 1st Edition. Used book that is in clean, average condition without any missing pages.
Published by Rutgers University Press, 2014
ISBN 10: 0813562465ISBN 13: 9780813562469
Seller: Night Heron Books, Laramie, WY, U.S.A.
Book
paperback. Condition: Very Good. Binding tight.Cover clean.Minor wear to page edges and corners. Paperback.No writing, highlighting, or marks in text.
Published by Birkhäuser, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: Hafa Adai Books, Moncks Corner, SC, U.S.A.
Book
Condition: very good.
Published by Birkhäuser, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: Books of the Smoky Mountains, Pflugerville, TX, U.S.A.
Book
Condition: very good. Gently used book with ongoing seller support until you're fully satisfied with your purchase.
Published by W. J. Gage Limited, Toronto, ON, Canada, 1965
Seller: Post Horizon Booksellers, Moose Jaw, SK, Canada
Book
Soft cover. Condition: Very Good. 120pp. Blackline illustrations. Titled green wrapper shows mild wear to back edges and front corners and school stamp on front cover. Stapled binding sound. This unmarked school workbook has not been written in. From the New Basic Readers Curriculum Foundation Series.
Published by Rutgers University Press, 2014
ISBN 10: 0813562465ISBN 13: 9780813562469
Seller: SGS Trading Inc, Franklin Lakes, NJ, U.S.A.
Book
Paperback. Condition: Good. Textbook, May Have Highlights, Notes and/or Underlining, BOOK ONLYNO ACCESS CODE, NO CD, Ships with Emailed Tracking.
Published by Birkhäuser, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: GoldBooks, Austin, TX, U.S.A.
Book
Paperback. Condition: new. New Copy. Customer Service Guaranteed.
Published by Birkhäuser, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: Wizard Books, Long Beach, CA, U.S.A.
Book
Paperback. Condition: new. New.
Published by Birkh?user, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: Front Cover Books, Denver, CO, U.S.A.
Book
Condition: new.
Published by Birkhäuser, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: GoldenWavesOfBooks, Fayetteville, TX, U.S.A.
Book
Paperback. Condition: new. New. Fast Shipping and good customer service.
Published by Springer, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: booksXpress, Bayonne, NJ, U.S.A.
Book
Soft Cover. Condition: new.
Published by Birkhäuser, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: Grumpys Fine Books, Tijeras, NM, U.S.A.
Book
Paperback. Condition: new. Prompt service guaranteed.
Published by Birkhäuser, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: BennettBooksLtd, LOS ANGELES, CA, U.S.A.
Book
Condition: New. New. In shrink wrap. Looks like an interesting title! 1.
Published by Rutgers University Press 2014-08-28, New Brunswick, 2014
ISBN 10: 0813562465ISBN 13: 9780813562469
Seller: Blackwell's, Oxford, OX, United Kingdom
Book
paperback. Condition: New. Language: ENG.
Published by Birkhauser Boston Inc, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: THE SAINT BOOKSTORE, Southport, United Kingdom
Book Print on Demand
Paperback / softback. Condition: New. This item is printed on demand. New copy - Usually dispatched within 5-9 working days.
Published by Birkhuser, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: Tacoma Book Center, Tacoma, WA, U.S.A.
Book
Paperback. Condition: Very Good. Dust Jacket Condition: No Dustjacket. Later Edition. ISBN 0817636439. Trade Paperback. Later Printing. Good Condition. Tight sound unmarked copy with slight smoky odor due to previous owner being a smoker, minor rubs to edges and corners of covers. No Signature.
Published by Birkhäuser Boston, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: AHA-BUCH GmbH, Einbeck, Germany
Book
Taschenbuch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations - Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat - Radiation - Toxic gas - Filtration - Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions - water as a reagent Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells - Common enzyme solutions - Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer - Particle counter - Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi - Fungi - Mycoplasma - Viruses - Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Inadequate cell growth - Recurrent contamination - When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards - Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum - Treatments of serum - Plasma-derived serum - Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 Coatingplasticware with solutions - Alterations with polymers - Using cells to coat the plasticware - Culturing cells on microcarriers Altering the Environment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 Temperature changes - Gaseous changes Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 CONTENTS vii PRIMARY CELL CULTURE. . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 Isolation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 Dissection - Enzymatic dissociation methods - Nonenzymatic isolation - Purification of cell suspensions - Consideringyield and survival Chatacterization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Published by Birkhäuser Boston, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: moluna, Greven, Germany
Book Print on Demand
Kartoniert / Broschiert. Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Published by Birkhäuser, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: Mispah books, Redhill, SURRE, United Kingdom
Book
Paperback. Condition: Like New. Like New. book.
Published by Birkhäuser Boston Aug 1994, 1994
ISBN 10: 0817636439ISBN 13: 9780817636432
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
Book Print on Demand
Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations - Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat - Radiation - Toxic gas - Filtration - Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions - water as a reagent Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells - Common enzyme solutions - Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer - Particle counter - Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi - Fungi - Mycoplasma - Viruses - Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Inadequate cell growth - Recurrent contamination - When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards - Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum - Treatments of serum - Plasma-derived serum - Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 Coatingplasticware with solutions - Alterations with polymers - Using cells to coat the plasticware - Culturing cells on microcarriers Altering the Environment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 Temperature changes - Gaseous changes Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 CONTENTS vii PRIMARY CELL CULTURE. . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 Isolation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 Dissection - Enzymatic dissociation methods - Nonenzymatic isolation - Purification of cell suspensions - Consideringyield and survival Chatacterization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 264 pp. Englisch.